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CRISPR-CAS9: Genome editing technology!

Post new topic   Reply to topic Forum Index -> News in Healthy Ageing during Andropause
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Joined: Jul 09, 2006
Posts: 621
Location: Manassas, Virginia, USA

PostPosted: Mon Aug 07, 2017 9:06 am    Post subject: CRISPR-CAS9: Genome editing technology! Reply with quote

gRNA constructs for CRISPR/Cas9-mediated genome editing
CRISPR/Cas9 Plasmid Reagents for sgRNA Expression & Gene Editing

CRISPR gRNA constructs
For the months of July and August: 20% Off CRISPR Plasmids
CRISPR/Cas9-mediated gene editing is a powerful technique that allows you to create knock-in/out mutations in any gene and any cell. CRISPR/Cas9 is advantageous over other forms of gene editing, such as TALENs and zinc finger nucleases, because it is simpler to implement and edits at higher efficiency.

GenScript licenses CRISPR technology frodeveloped by the CRISPR pioneering Feng Zhang laboratory. Broad Institute-validated plasmids are a well-tested platform for expressing CRISPR/Cas9, and avoid instability issues in RNA-based m the Broad Institute of MIT and Harvard. Our offerings include the latest CRISPR plasmids and databases platforms.

GenScript CRISPR Plasmid Repository

GenScript maintains a collection of more than 20,000 lentiCRISPRv2 plasmids containing guide RNA (gRNA) sequences pre-validated by the Broad Institute. Plasmids can be searched by gene name, symbol or ID on our gRNA Database.

Product Vector Selection Pricing  
GenCRISPR Plasmid Collection Lentiviral Amp, Puro $99 gRNA Database
SpCas9 Plasmids

Cas9 endonuclease is the research standard for gene editing. When combined with single guide RNA (sgRNA) sequences, these enzymes create site-specific double strand breaks (DSBs) in the genome. More information

Product Vector Selection Pricing
SpCas9 Plasmids Plasmid
Amp, Puro
Amp, Neo
Amp, GFP $199 Order
SpCas9 Nickase Plasmids

SpCas9 nickase (Cas9n D10A) contains a mutation allowing the endonuclease to create single-strand nicks, as opposed to DSBs. Pairing two opposite facing gRNA sequences with SpCas9 nickase is an efficient method of gene editing that prevents unwanted indels from forming.

Product Vector Selection Pricing
SpCas9 Nickase Plasmids Plasmid
Lentiviral Amp
Amp, Puro
Amp, GFP $199 Order
SaCas9 Plasmids

The Staphylococcus aureus Cas9 orthologue (SaCas9) is the preferred endonuclease for adeno-associated virus (AAV) applications. SaCas9 is approximately 1kb shorter than SpCas9, and offers additional flexibility around AAV packaging constraints. The lower immunogenicity of AAV vectors, makes SaCas9 well-suited for in vivoediting applications and therapeutics. More information

Product Vector Selection Pricing
SpCas9 Plasmids AVV Amp $199 Order
Transcription Activation (SAM) Plasmids

The CRISPR/Cas9 Synergistic Activation Mediator (SAM) system has been engineered to enable transcriptional activation of downstream targets. The SAM system utilizes three different activators, VP64, P65, and HSF1, which are assembled onto a catalytically dead Cas9 (dCas9) complex to drive transcription. More information

Product Vector Selection Pricing
SAM gRNA Plasmids Plasmid
Lentiviral Amp
Amp, Zeo $199 Order
SAM dCas9-VP64 Plasmids Lentiviral Amp, Blast
Amp, GFP $50 Order
SAM MS2-P65-HSF1 Plasmids Lentiviral Amp, GFP
Amp, Hygro $50 Order
Broad Institute Plasmid Collection

Broad Institute Plasmids are generated by the Broad Institute of Harvard and MIT. These plasmids contain a 17bp-1.8kb expressible linker in lieu of a customized sgRNA sequence, which can be modified by your laboratory.

Product Vector Selection Pricing
Broad Plasmid Collection Plasmid
Lentiviral Amp, Puro
Amp, GFP $99 Order
SpCas9 Nickase
Broad Plasmid Collection Plasmid Amp
Amp, Puro
Amp, GFP $99 Order
Broad Plasmid Collection AAV Amp $99 Order
Have questions? GenScript's Ph.D.-level service representatives are available 24 hours a day, Monday through Friday, to assist you.

1-877-436-7274 (Toll-Free)
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